Combined optimization of N-terminal site-specific PEGylation of recombinant hirudin using response surface methodology and kinetic analysis
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چکیده
منابع مشابه
Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
متن کاملpreparation and characterization of new co-fe and fe-mn nano catalysts using resol phenolic resin and response surface methodology study for fischer-tropsch synthesis
کاتالیزورهای co-fe-resol/sio2و fe-mn-resol/sio2 با استفاده از روش ساده و ارزان قیمت همرسوبی تهیه شدند. از رزین پلیمری resol در فرآیند تهیه کاتالیزور استفاده شد.
Optimization and High Level Production of Recombinant Synthetic Streptokinase in E. coli Using Response Surface Methodology
Streptokinase (SK) is an extracellular protein comprising 414 amino acids with considerable clinical importance as a commonly used thrombolytic agent. Due to its wide spread application and clinical importance designing more efficient SK production platforms worth investigatinginvestigation. In this regard, a synthetic SK gene was optimized and cloned in to pET21b plasmid for periplasmic expres...
متن کاملPhotocatalytic Removal of Amaranth Optimization Using Response Surface Methodology
Since Amaranth (AM) is one of the dye compounds which is harmful to human’s life its removal from industrial waste water would reduce their environmental impact and health effect. Copper nanoparticle (CuNP) is a simple and eco-friendly material which can be used to remove this pollutant. In this paper, copper nanoparticles were synthesized, for removal of AM dye. The experiments were designed b...
متن کاملSite-Specific PEGylation of Recombinant Immunotoxin DAB389IL-2: Structural and Functional Assessment
Background: DAB389IL-2 is considered a fusion immunotoxin and it is used for the CTCL therapy. DAB389IL-2 includes of two distinct portions; the catalytic domain of diphtheria toxin and IL-2. DAB389IL-2 duo to the presence of a free cysteine residue (Cys 513 in IL-2 part) is prone to unwanted intramolecular and intermolecular disulfide bonds formation and aggregation problems. Aggregation is co...
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ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2018
ISSN: 1618-0240
DOI: 10.1002/elsc.201700190